Tools for the genetic manipulation ofZygosaccharomyces rouxii

Development of Genetic Tools for the Manipulation of the Planctomycetes

Bacteria belonging to the Planctomycetes, Verrucomicrobia, Chlamydiae (PVC) superphylum are of interest for biotechnology, evolutionary cell biology, ecology, and human health. Some PVC species lack a number of typical bacterial features while others possess characteristics that are usually more associated to eukaryotes or archaea. For example, the Planctomycetes phylum is atypical for the abse...

Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei.

Because of Burkholderia pseudomallei's classification as a select agent in the United States, genetic manipulation of this bacterium is strictly regulated. Only a few antibiotic selection markers, including gentamicin, kanamycin, and zeocin, are currently approved for use with this bacterium, but wild-type strains are highly resistant to these antibiotics. To facilitate routine genetic manipula...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation

Intuitive tools for camera manipulation

We present an image-space camera manipulation widget that supports visualization of the relationship of the camera with respect to the scene. The form of the widget presents the user with natural affordances for camera manipulation. Visual aids such as ghosting of the scene and preview animations are used to acquaint novice users with the functions of different parts of the widget. Mouse gestur...

Plasmid Vectors and Molecular Building Blocks for the Development of Genetic Manipulation Tools for Trypanosoma cruzi

The post genomic era revealed the need for developing better performing, easier to use and more sophisticated genetic manipulation tools for the study of Trypanosoma cruzi, the etiological agent of Chagas disease. In this work a series of plasmids that allow genetic manipulation of this protozoan parasite were developed. First of all we focused on useful tools to establish selection strategies ...